การเกิดต้นจากการเพาะเลี้ยงแคลลัสของขมิ้นชันในหลอดทดลอง

Abstract

Buds of rhizome and sprout of Curcuma longa L. which collected from Bantakun and Thachana district
were cultured under aseptic condition to induce callus formation. The MS culture media containing various
concentrations of 2, 4-D or NAA or BAP were used. The results showed that various types of callus were achieved
depending upon types and plant growth regulator ratios in the culture media. Only the pale brown callus was
capable subculture to extend the number of callus masses. When the callus transferred to modified MS regeneration
media, numerous green nodes occurred and subsequently developed to shoots. Maximum plant regeneration was
achieved on the medium supplemented with 0.1 mg/l NAA + 3.0 mg/l BAP (15.8 plants, turmeric collected from
Bantakhun and 11.9 plants, turmeric collected from Thachana, respectively). Prolific roots of shoots were obtained
when the shoots were transferred to MS culture medium containing 1.0 or 3.0 mg/l NAA. High survival of plantlets
was attained after acclimatization in potted-soils covering with transparent plastic bags. Morphological observations
of callus derived plantlets did not differ to maternal plant.

 in-vitro-plant-regeneration-via-callus-culture-in-turmeric-curcuma-longa-l