Abstract

Effect of ethyl methanesulphonate (EMS) on mutation induction of Dendrobium Sonia was investigated in this study. The results showed that PLBs immersed in EMS at concentration of 0 and 0.75% for 60 and 90 minutes gave survival rate more than 50%. เท addition, PLBs immersed in EMS at concentration of 0.75% for 90 minutes showed the dark green and succulent PLBs while control treatment (without ISMS) gave light green and nonsucculent PLBs. After culture for further 12 months the plantlets obtained from treated PLBs with EMS at 0.75% for 90 minutes gave the highest growth rate with bigger leaves and thicker roots. The PLBs treated with EMS at 0.75% for 90 minutes also showed larger size of guard cells and lower in stomatal density than the control. Acclimatization of complete plantlets using peat moss gave the highest survival rate of plantlets at 91%

ethyl-methenesulphonate-EMS-Effect-of-ethyl-methanesulphonate-EMS-on-Dendrobium-Sonia