A pollen morphological study of banana cultivar (Musa acuminata Colla) in Surat Thani Province conducted from March 2015 to February 2016. The pollen size and exine sculpturing of 8 cultivars of banana were studied by light and field emission scanning electron microscopy. The 8 pollen samples were acetolyzed following the technique of Erdtman (1969). The pollen is spheroidal. In addition, the aperture types are colporate. The numbers of aperture found were one aperture. The exine sculpturing is psilate. The length of pollen grains in average was 45-122µm. Pollen size of banana cultivar genome B was larger than of genome A whereas triploid banana cultivar could be produced larger pollen size compared to diploid. The pollen size is differing among the banana cultivars. Therefore, the pollen morphology could be usefully identified banana cultivars.