Khao Dawk Pa-yawm Rice is native rice and suitable for growing in the Southern part of Thailand. Currently, the rice fields are in decline. The experiment was study of micro propagation for the conservation of genetic further. Callus induction from seed in vitro cultured on MS, ½MS and N6 media for 4 weeks. The results showed that the callus formation were 75.56%, 62.23% and 46.67%, respectively. However, The largest callus was found on the ½MS medium with average size is 1.12 cm. Increasing the amount of callus by cultured its on ½MS medium and addition of three types of growth regulators are 2,4-D, NAA and BA at various concentrations was done for 4 weeks. It was found that medium supplemented with 1 mg/L and 1.5 mg/L of growth regulators gave the highest callus proliferation. The maximum weight is 1.03 and 0.93 g, respectively. For plant regeneration, the cultured on MS medium supplemented with BAP, Kin and NAA difference concentrations was conducted for 4 weeks. The results showed that MS medium supplemented the combination 2.0 mg/L BAP with 0.5 mg/L Kin and 0.5 mg/L NAA was regenerated the plant average 20% of shoot formation
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