Biosurfactants-producing bacteria were isolated from various palm oil refinery industrial sites in the south of Thailand. Isolates were screened for biosurfactant production by using low-cost, agro-industrial by-products or wastes as a substrate. Based on drop collapsing test and emulsification activity, 25 isolates were selected. All the selected isolates reduced the growth medium surface tension to 40 mN/m and produced emulsions with xylene. Twenty isolates exhibited high emulsion-stabilizing capacity, maintaining more than 50% of the original emulsion volume for 24 h. The phylogenetic position of these 25 isolates was evaluated by 16S rRNA gene sequence analysis.The production of biosurfactants was determined for strains representative of 15 different bacterial genera, six of them (Azorhizobium, Buttiauxella, Comamonas, Halopenitus, Haloplanus and Sinorhizobium ) have been for the first time reported in this study as biosurfactant-producing strains. Additionally, Sinorhizobium meliloti AS91 and Marinobacter hydrocarbonoclasticus AS51 produced extracellular biosurfactant which exhibited the lowest surface tension (32 mN/m) and emulsification activity (69%) when cashew apple juice and used vegetable oil were used as the carbon source, respectively. Overall, this is the first study of a phylogenetic analysis of biosurfactant-producing bacteria from palm oil refinery industry site and their ability to produce biosurfactant on renewable substrates.
Welcome
Business hours 08:30 - 16:30 on business days and hours. Saturday-Sunday from 08:30 - 16:30 according to the academic calendar.
The Dean of the Faculty of Science and Technology's office is located in the Faculty of Science and Technology building. Surat Thani Rajabhat University 272 Moo 9, Khun Thale Subdistrict, Mueang District, Surat Thani Province 84100.
