Abstract

Mature  leaves  of Torenia  (Torenia  fournieriLind.)  were  cultured  on  MS  (Murashige  and Skoog,  1962)  medium  supplemented  with  various  concentrations  of  NAA  (-naphthalene  acetic acid) and BAP (N6-benzylaminopurine). Calluses, shoots, roots and somatic embryos occurred in different media, depending upon the ratios and concentrations of NAA and BAP. In the medium containing BAP alone (1.0, 3.0, 5.0 mg/l), explants were proliferated shoots reach to 100%. The maximum  shoots  formation  was  obtained  in  the  medium  containing  5.0  mg/l  BAP  (55.5±1.2).Whereas somatic embryos were formed on the media containing 1.0, 3.0 and 5.0 mg/l BAPplus 0.1  or  0.5  mg/l  NAA.  The  highest  somatic  embryos  production  was  occurred  on  the  medium containing both 5.0 mg/l BAP and 0.1 mg/l NAA (59.2±3.7). The media containing NAA singly (0.1, 1.5, 3.0 mg/l) resulted in explants rich in roots and calluses. Morphogenesis monitoring under the microscopes displayed that somatic embryos were formed in the media containing 0.1 or 0.5 mg/l NAA plus 3.0 or 5.0 mg/l BAP and significantly confirmed that the upper leaf side was the origin of the  shoots.  The  somatic  embryos  occurred  without  intervening  callus  phse.  Somatic  embryos automatically developed to seedlings without changing the medium. Shoots were rooted in the MS  medium  containing  200 mg/l  activated  charcoal. In  vitroderived  plants  were  acclimatized successfully and grew with uniformity as mother plant

somatic-embryo-development-and-maturation-of-mature-leaf-blossom